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ecori sali fragment  (TaKaRa)


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    Structured Review

    TaKaRa ecori sali fragment
    Ecori Sali Fragment, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 16004 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecori+sali+fragment/EcoR+I/pm40093512-130-12-27
    Average 99 stars, based on 16004 article reviews
    ecori sali fragment - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Expressing:

    Article Title: Mutation of TRIM28-Lys304 to Gln Attenuates its Interaction with KRAB-ZNFs and Promotes Differentiation of Erythroleukemic K562 Cells
    Article Snippet: .. The EcoRI-SalI fragment from FLAG-Trim28 was ligated into pCMV-HA-N (Clontech) for HA-Trim28 expression. .. The TRIM28 mutants were generated with the Q5 SiteDirected Mutagenesis kit (NEB) and primers shown in Table S4A.

    Article Title: Anti-lymphoma peptide is inspired by mapping a sequence of four amino acids of KRAI motif as nuclear localization signal of Crlz-1.
    Article Snippet: 33 March 2025 was filled using Klenow enzyme and subcloned into the EcoRV site of pCMV-Tag2A (Stratagene) in frame with the codons of FLAG tag peptide. .. Then, the GFP-fused Crlz-1 mammalian expression plasmid was constructed by inserting the EcoRI-SalI fragment of the above pCMV-Tag2A-based Crlz-1 expression plasmid into the corresponding sites of pEGFP-C1 (Clontech). .. Various Crlz-1 truncations, deletions, and site-directed mutations with GFP at their 50 sides were made by using various molecular biological techniques and a sitedirected mutagenesis kit (Clontech) with care taken to maintain their coding frames.

    Article Title: Acetylation-Mimic Mutation of TRIM28-Lys304 to Gln Attenuates the Interaction with KRAB-Zinc-Finger Proteins and Affects Gene Expression in Leukemic K562 Cells
    Article Snippet: .. The EcoRI-SalI fragment from FLAG-Trim28 was ligated into pCMV-HA-N (Clontech, Mountain View, CA, USA) for HA-Trim28 expression. .. The TRIM28 mutants were generated with the Q5 Site-Directed Mutagenesis kit (New England Biolabs, Ipswich, MA, USA) and primers shown in .

    Article Title: Anti-lymphoma peptide is inspired by mapping a sequence of four amino acids of KRAI motif as nuclear localization signal of Crlz-1
    Article Snippet: For the construction of wt full-length Crlz-1 cDNA mammalian expression plasmid, the NcoI-NotI cDNA fragment taken from the plasmid of an isolated λ phage clone was filled using Klenow enzyme and subcloned into the EcoRV site of pCMV-Tag2A (Stratagene) in frame with the codons of FLAG tag peptide. .. Then, the GFP -fused Crlz-1 mammalian expression plasmid was constructed by inserting the EcoRI-SalI fragment of the above pCMV-Tag2A-based Crlz-1 expression plasmid into the corresponding sites of pEGFP-C1 (Clontech). .. Various Crlz-1 truncations, deletions, and site-directed mutations with GFP at their 5′ sides were made by using various molecular biological techniques and a site-directed mutagenesis kit (Clontech) with care taken to maintain their coding frames.

    Article Title: Acetylation-Mimic Mutation of TRIM28-Lys304 to Gln Attenuates the Interaction with KRAB-Zinc-Finger Proteins and Affects Gene Expression in Leukemic K562 Cells.
    Article Snippet: .. The EcoRI-SalI fragment from FLAG-Trim28 was ligated into pCMV-HA-N (Clontech, Mountain View, CA, USA) for HA-Trim28 expression. .. The TRIM28 mutants were generated with the Q5 Site-Directed Mutagenesis kit (New England Biolabs, Ipswich, MA, USA) and primers shown in Table S5A.

    Plasmid Preparation:

    Article Title: Anti-lymphoma peptide is inspired by mapping a sequence of four amino acids of KRAI motif as nuclear localization signal of Crlz-1.
    Article Snippet: 33 March 2025 was filled using Klenow enzyme and subcloned into the EcoRV site of pCMV-Tag2A (Stratagene) in frame with the codons of FLAG tag peptide. .. Then, the GFP-fused Crlz-1 mammalian expression plasmid was constructed by inserting the EcoRI-SalI fragment of the above pCMV-Tag2A-based Crlz-1 expression plasmid into the corresponding sites of pEGFP-C1 (Clontech). .. Various Crlz-1 truncations, deletions, and site-directed mutations with GFP at their 50 sides were made by using various molecular biological techniques and a sitedirected mutagenesis kit (Clontech) with care taken to maintain their coding frames.

    Article Title: Anti-lymphoma peptide is inspired by mapping a sequence of four amino acids of KRAI motif as nuclear localization signal of Crlz-1
    Article Snippet: For the construction of wt full-length Crlz-1 cDNA mammalian expression plasmid, the NcoI-NotI cDNA fragment taken from the plasmid of an isolated λ phage clone was filled using Klenow enzyme and subcloned into the EcoRV site of pCMV-Tag2A (Stratagene) in frame with the codons of FLAG tag peptide. .. Then, the GFP -fused Crlz-1 mammalian expression plasmid was constructed by inserting the EcoRI-SalI fragment of the above pCMV-Tag2A-based Crlz-1 expression plasmid into the corresponding sites of pEGFP-C1 (Clontech). .. Various Crlz-1 truncations, deletions, and site-directed mutations with GFP at their 5′ sides were made by using various molecular biological techniques and a site-directed mutagenesis kit (Clontech) with care taken to maintain their coding frames.

    Construct:

    Article Title: Anti-lymphoma peptide is inspired by mapping a sequence of four amino acids of KRAI motif as nuclear localization signal of Crlz-1.
    Article Snippet: 33 March 2025 was filled using Klenow enzyme and subcloned into the EcoRV site of pCMV-Tag2A (Stratagene) in frame with the codons of FLAG tag peptide. .. Then, the GFP-fused Crlz-1 mammalian expression plasmid was constructed by inserting the EcoRI-SalI fragment of the above pCMV-Tag2A-based Crlz-1 expression plasmid into the corresponding sites of pEGFP-C1 (Clontech). .. Various Crlz-1 truncations, deletions, and site-directed mutations with GFP at their 50 sides were made by using various molecular biological techniques and a sitedirected mutagenesis kit (Clontech) with care taken to maintain their coding frames.

    Article Title: Anti-lymphoma peptide is inspired by mapping a sequence of four amino acids of KRAI motif as nuclear localization signal of Crlz-1
    Article Snippet: For the construction of wt full-length Crlz-1 cDNA mammalian expression plasmid, the NcoI-NotI cDNA fragment taken from the plasmid of an isolated λ phage clone was filled using Klenow enzyme and subcloned into the EcoRV site of pCMV-Tag2A (Stratagene) in frame with the codons of FLAG tag peptide. .. Then, the GFP -fused Crlz-1 mammalian expression plasmid was constructed by inserting the EcoRI-SalI fragment of the above pCMV-Tag2A-based Crlz-1 expression plasmid into the corresponding sites of pEGFP-C1 (Clontech). .. Various Crlz-1 truncations, deletions, and site-directed mutations with GFP at their 5′ sides were made by using various molecular biological techniques and a site-directed mutagenesis kit (Clontech) with care taken to maintain their coding frames.

    Polymerase Chain Reaction:

    Article Title: Tumor endothelial cell-derived Sfrp1 supports the maintenance of cancer stem cells via Wnt signaling.
    Article Snippet: .. The PCR product containing the EcoRI/SalI fragment was inserted into the pIRES-EGFP vector (Clontech, Mountain View, CA). ..

    Article Title: Tumor endothelial cell-derived Sfrp1 supports the maintenance of cancer stem cells via Wnt signaling
    Article Snippet: .. The PCR product containing the EcoRI/SalI fragment was inserted into the pIRES-EGFP vector (Clontech, Mountain View, CA). ..

    Generated:

    Article Title: A novel role for atypical MAPK kinase ERK3 in regulating breast cancer cell morphology and migration
    Article Snippet: .. EGFP-ERK3 (GFP-ERK3) was generated by cloning the EcoRI-SalI fragment from pGBKT7-ERK3. in the corresponding sites of pEGFP-C1 (Clontech). .. The zebrafish ERK3 was amplified from the image clone (IMAGE:9038031) using primers 5′- ggcgaattcatcacagaatggcagagaaatttgaaagc-3 ́and 5 ́- gcggatccttacttatcgtcgtcatccttgtaatcatttaaatgcttgaaaatgctgc -3 and subcloned as an EcoRI-BamHI fragment into pSG5 vector (Stratagene) generating pSG5-Flag-zfERK3.

    Cloning:

    Article Title: A novel role for atypical MAPK kinase ERK3 in regulating breast cancer cell morphology and migration
    Article Snippet: .. EGFP-ERK3 (GFP-ERK3) was generated by cloning the EcoRI-SalI fragment from pGBKT7-ERK3. in the corresponding sites of pEGFP-C1 (Clontech). .. The zebrafish ERK3 was amplified from the image clone (IMAGE:9038031) using primers 5′- ggcgaattcatcacagaatggcagagaaatttgaaagc-3 ́and 5 ́- gcggatccttacttatcgtcgtcatccttgtaatcatttaaatgcttgaaaatgctgc -3 and subcloned as an EcoRI-BamHI fragment into pSG5 vector (Stratagene) generating pSG5-Flag-zfERK3.



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